A universal, vector-based system for nucleic acid reading-frame selection

A universal, vector-based system for nucleic acid reading-frame selection.

The identification of a nucleic acid sequence's correct reading frame has important implications for homology-independent protein engineering techniques such as incremental truncation and SCRATCHY. We report the development and experimental implementation of a general in-frame selection system, pSALect, a plasmid vector that utilizes two marker sequences flanking the DNA of interest. This dual ...

A second-generation system for unbiased reading frame selection.

Reading frame selection of nucleic acids has important implications for protein engineering and genomics. Current methods are limited because selection of the gene of interest inevitably depends on the solubility of its translated product. Here we report the construction of the pInSALect vector, which provides strict reading frame selection without concomitant selection for protein solubility o...

Efficient frame vector selection based on ordered sets

The problem of finding the optimal set of quantized coefficients for a frame-based encoded signal is known to be of very high complexity. This paper presents an efficient method of finding the operational Rate-Distortion (RD) optimal set of coefficients. The major complexity reduction lies in the reformulation of the original RD-tradeoff problem, where a new set of coefficients is used as decis...

Sensitivity and Specificity of Nucleic Acid Sequence-Based Amplification Method for Diagnosis of Cutaneous Leishmaniasis

Abstract Background and Objective: Culture, microscopic method is a gold standard method for identification of Lishmania parasite. The use of Molecular methods such as RT- PCR compared to microscopic methods has a higher sensitivity and specificity however, it is not widely used due to its expensive equipment and the time requested. The use of nucleic acid sequence based amplification (NASBA) ...

Preparation of SCRATCHY hybrid protein libraries: size- and in-frame selection of nucleic acid sequences.